Hotspot

trnK-UUU-rps16

LSC · 4627-5735 · 1109 bp

Back to Rhus

Pi 0.0211
MarkerSeek score 67.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1109
variable_sites
59
pi
0.0211
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.96
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGGGGTTTTTACGCAGCTTA 50.0 50.0 58.2 58.5 1042-1189 bp 1.0 83.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGGGGTTTTTACGCAGCTTA 50.0 50.0 58.2 58.5 1041-1188 bp 1.0 83.1
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA GGGGGTTTTTACGCAGCTTA 50.0 50.0 58.3 58.5 1051-1198 bp 1.0 82.1
trnK-UUU-rps16_p4 TACCGTTGAGTTAGCAACCC GGGGGTTTTTACGCAGCTTA 50.0 50.0 57.5 58.5 1028-1175 bp 1.0 80.8
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GGGGTTTTTACGCAGCTTAG 50.0 50.0 58.2 57.1 1040-1187 bp 1.0 79.5