Hotspot

rpl27-rpl21

Genome · 110382-110392 · 11 bp

Back to Rhopalodia

Pi 0.0606
MarkerSeek score 78.20
Primer available yes
Label rpl27-rpl21

Feature Metadata

label_name
rpl27-rpl21
feature_type
igs
region
Genome
length_bp
11
variable_sites
1
pi
0.0606
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl27-rpl21_p1 CTTTTTACGCCAAGCCGTTT GCTGTTAGTCTCGGCTTAGG 45.0 55.0 57.9 57.8 676-677 bp 1.0 80.7
rpl27-rpl21_p2 CCAAGCCGTTTTGCATTTGA GCTGTTAGTCTCGGCTTAGG 45.0 55.0 58.1 57.8 667-668 bp 1.0 80.7
rpl27-rpl21_p3 CTTTTTACGCCAAGCCGTTT AAACAGCTGTTAGTCTCGGC 45.0 50.0 57.9 58.2 682 bp 0.333333 50.7
rpl27-rpl21_p4 CCAAGCCGTTTTGCATTTGA AAACAGCTGTTAGTCTCGGC 45.0 50.0 58.1 58.2 673 bp 0.333333 50.6
rpl27-rpl21_p5 CCACCAAACCTTTTTACGCC AAACAGCTGTTAGTCTCGGC 50.0 50.0 57.9 58.2 691 bp 0.333333 50.6