Hotspot

ccsA-psbA

Genome · 102796-103207 · 412 bp

Back to Rhopalodia

Pi 0.118

MarkerSeek score 80.70

Primer available yes

Label ccsA-psbA

Feature Metadata

label_name: ccsA-psbA
feature_type: igs
region: Genome
length_bp: 412
variable_sites: 69
pi: 0.118
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psbA_p1	CGTGGTTGGTTTTTGCAACT	TTCTCAGCTATGCACGGTTC	45.0	50.0	58.0	58.0	1013-1029 bp	0.666667	69.8
ccsA-psbA_p2	CGTGGTTGGTTTTTGCAACT	TCGTATTCCAAGCTGAGCAC	45.0	50.0	58.0	58.0	1093-1109 bp	0.666667	69.5
ccsA-psbA_p3	CGTGGTTGGTTTTTGCAACT	ATTCCAAGCTGAGCACAACA	45.0	45.0	58.0	58.0	1089-1105 bp	0.666667	69.5
ccsA-psbA_p4	CGTGGTTGGTTTTTGCAACT	TCCAAGCTGAGCACAACATT	45.0	45.0	58.0	58.0	1087-1103 bp	0.666667	69.5
ccsA-psbA_p5	CGTGGTTGGTTTTTGCAACT	TTCCAAGCTGAGCACAACAT	45.0	45.0	58.0	58.0	1088-1104 bp	0.666667	69.5