Hotspot

rps16-trnL-UAA

Genome · 96157-96635 · 479 bp

Back to Rhopalodia

Pi 0.146

MarkerSeek score 82.70

Primer available yes

Label rps16-trnL-UAA

Feature Metadata

label_name: rps16-trnL-UAA
feature_type: igs
region: Genome
length_bp: 479
variable_sites: 90
pi: 0.146
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnL-UAA_p1	TAAAAGAAGCACGTCGCAGA	GGTGGAATGGTAGACACGAC	45.0	55.0	57.9	58.0	1200-1280 bp	0.666667	71.7
rps16-trnL-UAA_p2	TAAAAGAAGCACGTCGCAGA	ATTGCAAATCCTCGACCCTC	45.0	50.0	57.9	58.0	1318-1398 bp	0.666667	71.3
rps16-trnL-UAA_p3	AACGGGTTGTAAGCATACCC	GGTGGAATGGTAGACACGAC	50.0	55.0	57.9	58.0	1127 bp	0.333333	52.3
rps16-trnL-UAA_p4	AACGGGTTGTAAGCATACCC	ATTGCAAATCCTCGACCCTC	50.0	50.0	57.9	58.0	1245 bp	0.333333	52.0
rps16-trnL-UAA_p5	AACGGGTTGTAAGCATACCC	GATTGCAAATCCTCGACCCT	50.0	50.0	57.9	58.0	1246 bp	0.333333	52.0