Hotspot

rrn5-ycf35

Genome · 70744-71518 · 775 bp

Back to Rhopalodia

Pi 0.101

MarkerSeek score 83.70

Primer available yes

Label rrn5-ycf35

Feature Metadata

label_name: rrn5-ycf35
feature_type: igs
region: Genome
length_bp: 775
variable_sites: 104
pi: 0.101
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-ycf35_p1	CGACGACAATACTTGGAGGG	ACCTCGCCAGCATATTGTTT	55.0	45.0	58.1	57.9	1044-1105 bp	0.666667	71.8
rrn5-ycf35_p2	TTCGGTCCATATCCGGTGTA	ACCTCGCCAGCATATTGTTT	50.0	45.0	57.9	57.9	1514-1575 bp	0.666667	70.6
rrn5-ycf35_p3	ATCTCCCCCAAGAGTTCACA	ACCTCGCCAGCATATTGTTT	50.0	45.0	58.0	57.9	1659-1720 bp	0.666667	70.3
rrn5-ycf35_p4	TCTCCCCCAAGAGTTCACAT	ACCTCGCCAGCATATTGTTT	50.0	45.0	58.0	57.9	1658-1719 bp	0.666667	70.3
rrn5-ycf35_p5	TAGTGATCTGACGGTGCTGA	ACCTCGCCAGCATATTGTTT	50.0	45.0	58.2	57.9	1731-1792 bp	0.666667	69.2