Hotspot

ftsH-trnP-UGG

Genome · 63853-64317 · 465 bp

Back to Rhopalodia

Pi 0.0552

MarkerSeek score 80.00

Primer available yes

Label ftsH-trnP-UGG

Feature Metadata

label_name: ftsH-trnP-UGG
feature_type: igs
region: Genome
length_bp: 465
variable_sites: 38
pi: 0.0552
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ftsH-trnP-UGG_p1	ATGGTTTGCCCCAGAAGAAG	CCAAGAACACTTCCCACCAA	50.0	50.0	58.1	57.9	1406-1430 bp	1.0	84.2
ftsH-trnP-UGG_p2	GGACATGCAATTGTTGGCTC	CCAAGAACACTTCCCACCAA	50.0	50.0	58.0	57.9	1519 bp	0.666667	67.1
ftsH-trnP-UGG_p3	AATCGAACGCCAGGATTCTC	CCAAGAACACTTCCCACCAA	50.0	50.0	58.1	57.9	1714 bp	0.666667	66.7
ftsH-trnP-UGG_p4	CCGATCGTATTATTGGCGGT	CCAAGAACACTTCCCACCAA	50.0	50.0	57.9	57.9	1599 bp	0.333333	52.2
ftsH-trnP-UGG_p5	GGCGGTATTGCTGGTACTAC	CCAAGAACACTTCCCACCAA	55.0	50.0	58.1	57.9	1585 bp	0.333333	51.7