Hotspot

trnF-GAA-psbX

Genome · 28250-28852 · 603 bp

Back to Rhopalodia

Pi 0.121

MarkerSeek score 89.90

Primer available yes

Label trnF-GAA-psbX

Feature Metadata

label_name: trnF-GAA-psbX
feature_type: igs
region: Genome
length_bp: 603
variable_sites: 107
pi: 0.121
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 89.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-psbX_p1	GAACTGGTGACACGAGGATC	AAACCGCACCAATAGCAAGA	55.0	45.0	58.0	58.1	682-735 bp	1.0	91.2
trnF-GAA-psbX_p2	TCCACTGCTCTACCAACTGA	AAACCGCACCAATAGCAAGA	50.0	45.0	58.0	58.1	657-710 bp	1.0	91.2
trnF-GAA-psbX_p3	GATTCGAACTGGTGACACGA	AAACCGCACCAATAGCAAGA	50.0	45.0	57.9	58.1	687-740 bp	1.0	90.8
trnF-GAA-psbX_p4	AGATTCGAACTGGTGACACG	AAACCGCACCAATAGCAAGA	50.0	45.0	57.9	58.1	688-741 bp	1.0	90.7
trnF-GAA-psbX_p5	ATTCGAACTGGTGACACGAG	AAACCGCACCAATAGCAAGA	50.0	45.0	57.9	58.1	686-739 bp	1.0	90.7