Hotspot

trnfM-CAU-rpl12

Genome · 24552-24975 · 424 bp

Back to Rhopalodia

Pi 0.174
MarkerSeek score 82.10
Primer available yes
Label trnfM-CAU-rpl12

Feature Metadata

label_name
trnfM-CAU-rpl12
feature_type
igs
region
Genome
length_bp
424
variable_sites
105
pi
0.174
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-rpl12_p1 TACCAGACTGCTCTACTCCG ACAAAATCGTCCATCCGGAG 55.0 50.0 58.0 58.0 957-978 bp 1.0 89.5
trnfM-CAU-rpl12_p2 TACCAGACTGCTCTACTCCG CAAAATCGTCCATCCGGAGT 55.0 50.0 58.0 58.0 956-977 bp 1.0 89.5
trnfM-CAU-rpl12_p3 GGGCTTGTCCAGGTAATAGC ACAAAATCGTCCATCCGGAG 55.0 50.0 58.0 58.0 1198-1219 bp 1.0 89.1
trnfM-CAU-rpl12_p4 GGGCTTGTCCAGGTAATAGC CAAAATCGTCCATCCGGAGT 55.0 50.0 58.0 58.0 1197-1218 bp 1.0 89.1
trnfM-CAU-rpl12_p5 CTAAGCACTGTTCTTTGCGC CAAAATCGTCCATCCGGAGT 50.0 50.0 58.0 58.0 1292-1313 bp 1.0 88.9