Hotspot

trnD-GUC-petM

Genome · 17537-17834 · 298 bp

Back to Rhopalodia

Pi 0.134

MarkerSeek score 78.30

Primer available yes

Label trnD-GUC-petM

Feature Metadata

label_name: trnD-GUC-petM
feature_type: igs
region: Genome
length_bp: 298
variable_sites: 57
pi: 0.134
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-petM_p1	AGTGGTTAGAGCAGGGGATT	GGCTGTAGTTATGGCTGCAT	50.0	50.0	58.0	58.0	1098-1132 bp	1.0	88.7
trnD-GUC-petM_p2	AGTGGTTAGAGCAGGGGATT	ATGGCTGTAGTTATGGCTGC	50.0	50.0	58.0	58.0	1100-1134 bp	1.0	88.6
trnD-GUC-petM_p3	TGGTTAGAGCAGGGGATTCA	GGCTGTAGTTATGGCTGCAT	50.0	50.0	58.0	58.0	1096-1130 bp	1.0	88.6
trnD-GUC-petM_p4	AGTGGTTAGAGCAGGGGATT	TGGTCCAAATGCGGTGTAAA	50.0	45.0	58.0	58.0	1360-1394 bp	1.0	87.1
trnD-GUC-petM_p5	TGGTTAGAGCAGGGGATTCA	TGGTCCAAATGCGGTGTAAA	50.0	45.0	58.0	58.0	1358-1392 bp	1.0	87.0