Hotspot

trnK-UUU-psbC

Genome · 12645-12825 · 181 bp

Back to Rhopalodia

Pi 0.151

MarkerSeek score 80.30

Primer available yes

Label trnK-UUU-psbC

Feature Metadata

label_name: trnK-UUU-psbC
feature_type: igs
region: Genome
length_bp: 181
variable_sites: 40
pi: 0.151
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-psbC_p1	TGGGGTTGCTAACTCAATGG	AACCGTGCTAATGAACCTGT	50.0	45.0	57.8	57.4	294-300 bp	1.0	83.7
trnK-UUU-psbC_p2	GGGGTTGCTAACTCAATGGT	AACCGTGCTAATGAACCTGT	50.0	45.0	57.8	57.4	293-299 bp	1.0	83.7
trnK-UUU-psbC_p3	TAGTTCTGGGTTCGAGTCCC	AACCGTGCTAATGAACCTGT	55.0	45.0	58.4	57.4	250-256 bp	1.0	82.1
trnK-UUU-psbC_p4	ACCGAATAGTTCTGGGTTCG	AACCGTGCTAATGAACCTGT	50.0	45.0	57.3	57.4	256-262 bp	1.0	80.3
trnK-UUU-psbC_p5	CCGAATAGTTCTGGGTTCGA	AACCGTGCTAATGAACCTGT	50.0	45.0	57.0	57.4	255-261 bp	1.0	78.0