Hotspot

trnE-UUC-trnG-GCC

Genome · 11647-11681 · 35 bp

Back to Rhopalodia

Pi 0.101
MarkerSeek score 83.00
Primer available yes
Label trnE-UUC-trnG-GCC

Feature Metadata

label_name
trnE-UUC-trnG-GCC
feature_type
igs
region
Genome
length_bp
35
variable_sites
5
pi
0.101
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.94
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnG-GCC_p1 GAGCTGTCCTAGGCCTCTAG GGGCATAGCTCAGTGGTAGA 60.0 55.0 58.4 58.6 134-136 bp 1.0 76.4
trnE-UUC-trnG-GCC_p2 CTGTCCTAGGCCTCTAGACG GGGCATAGCTCAGTGGTAGA 60.0 55.0 58.4 58.6 131-133 bp 1.0 76.4
trnE-UUC-trnG-GCC_p3 CTAGGCCTCTAGACGATGGG GGGCATAGCTCAGTGGTAGA 60.0 55.0 58.5 58.6 126-128 bp 1.0 76.1
trnE-UUC-trnG-GCC_p4 CCTAGGCCTCTAGACGATGG GGGCATAGCTCAGTGGTAGA 60.0 55.0 58.5 58.6 127-129 bp 1.0 76.0
trnE-UUC-trnG-GCC_p5 TGAAAGGGAGCTGTCCTAGG GGGCATAGCTCAGTGGTAGA 55.0 55.0 58.4 58.6 141-143 bp 1.0 75.9