Hotspot

ycf1

SSC · 126547-131955 · 5409 bp

Back to Rhopaloblaste

Pi 0
MarkerSeek score 52.50
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5409
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 ACGCCATTAAATCCGTGTCA TGTTCCCAGAATTTGTGCCA 45.0 45.0 57.9 57.9 1159 bp 1.0 78.6
ycf1_p2 ACGCCATTAAATCCGTGTCA CCCAGAATTTGTGCCACTCT 45.0 50.0 57.9 58.1 1155 bp 1.0 78.5
ycf1_p3 ACGCCATTAAATCCGTGTCA TGTGCCACTCTTTGATGCAT 45.0 45.0 57.9 58.1 1146 bp 1.0 78.5
ycf1_p4 ACGCCATTAAATCCGTGTCA TCCCAGAATTTGTGCCACTC 45.0 50.0 57.9 58.1 1156 bp 1.0 78.5
ycf1_p5 ACGCCATTAAATCCGTGTCA TAGAACCCCAGTTGTTCCCA 45.0 50.0 57.9 58.2 1171 bp 1.0 77.8