Hotspot

rpl16

LSC · 83489-85047 · 1559 bp

Back to Rhopaloblaste

Pi 0
MarkerSeek score 58.30
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1559
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCAATGCAAAGGATGAAGGA AGGTTGAATAAAAATTCGATTGACCA 40.0 30.8 54.9 58.1 1706 bp 1.0 40.0
rpl16_p2 TCAATGCAAAGGATGAAGGA GGTTGAATAAAAATTCGATTGACCA 40.0 32.0 54.9 56.9 1705 bp 1.0 40.0
rpl16_p3 TCAATGCAAAGGATGAAGGAA AGGTTGAATAAAAATTCGATTGACCA 38.1 30.8 55.7 58.1 1706 bp 1.0 40.0
rpl16_p4 TTCAATGCAAAGGATGAAGGA AGGTTGAATAAAAATTCGATTGACCA 38.1 30.8 55.7 58.1 1707 bp 1.0 40.0
rpl16_p5 TCAATGCAAAGGATGAAGGAA GGTTGAATAAAAATTCGATTGACCA 38.1 32.0 55.7 56.9 1705 bp 1.0 40.0