Hotspot

psaC-ndhE

SSC · 120005-120469 · 465 bp

Back to Rhopaloblaste

Pi 0.0152
MarkerSeek score 64.70
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
465
variable_sites
7
pi
0.0152
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 ACATCCGTCCCAAGGTATCA CTGAAGCAGCTATTGGGCTA 50.0 50.0 58.1 57.4 659-663 bp 1.0 74.9
psaC-ndhE_p2 ACATCCGTCCCAAGGTATCA TGAAGCAGCTATTGGGCTAG 50.0 50.0 58.1 57.4 658-662 bp 1.0 74.9
psaC-ndhE_p3 ACATCCGTCCCAAGGTATCA TGTTATAGCTGTTGCGGCTG 50.0 50.0 58.1 58.6 679-683 bp 1.0 74.9
psaC-ndhE_p4 ACATCCGTCCCAAGGTATCA GAAGCAGCTATTGGGCTAGC 50.0 55.0 58.1 58.8 657-661 bp 1.0 73.8
psaC-ndhE_p5 CAATACATCTGTGGGGCAGG TGAAGCAGCTATTGGGCTAG 55.0 50.0 58.3 57.4 634-638 bp 1.0 73.5