Hotspot

ndhF-rpl32

SSC · 115550-115827 · 278 bp

Back to Rhopaloblaste

Pi 0
MarkerSeek score 52.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
278
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCTGTCGGAATAAGT GGTGGAAACCGATTTCGCTA 40.0 50.0 55.3 58.3 497-498 bp 1.0 56.4
ndhF-rpl32_p2 TTTGTTGCTGTCGGAATAAGT GGTGGAAACCGATTTCGCTA 38.1 50.0 56.1 58.3 498-499 bp 1.0 54.8
ndhF-rpl32_p3 TTTTGTTGCTGTCGGAATAAGT GGTGGAAACCGATTTCGCTA 36.4 50.0 56.8 58.3 499-500 bp 1.0 52.6
ndhF-rpl32_p4 TTTTTGTTGCTGTCGGAATAAGT GGTGGAAACCGATTTCGCTA 34.8 50.0 57.5 58.3 500-501 bp 1.0 49.9
ndhF-rpl32_p5 TGTTGCTGTCGGAATAAGTA GGTGGAAACCGATTTCGCTA 40.0 50.0 54.4 58.3 496-497 bp 1.0 49.4