Hotspot

accD-psaI

LSC · 60769-61641 · 873 bp

Back to Rhopaloblaste

Pi 0
MarkerSeek score 58.50
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
873
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CCACGGTTTCTTTCCCTTGA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 986-991 bp 1.0 79.6
accD-psaI_p2 TCCACGGTTTCTTTCCCTTG CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 987-992 bp 1.0 79.6
accD-psaI_p3 CCACGGTTTCTTTCCCTTGA AAGAAGCCATTGCAATTGCC 50.0 45.0 58.0 57.9 992-997 bp 1.0 79.0
accD-psaI_p4 TCCACGGTTTCTTTCCCTTG AAGAAGCCATTGCAATTGCC 50.0 45.0 58.0 57.9 993-998 bp 1.0 79.0
accD-psaI_p5 TCAGCTCCACGGTTTCTTTC CCATTGCAATTGCCGGAAAT 50.0 45.0 58.1 58.0 992-997 bp 1.0 78.8