primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	TCAACGATTCATATACACTAAGACA	TGAGATTGGTTGACATTGGCA	25	21	32.0	42.9	55.9	58.1	0.0	0.0	8.201571	241	1344	1223-1223	1.000000	40.7
psbA_p2	psbA	2	CAACGATTCATATACACTAAGACA	TGAGATTGGTTGACATTGGCA	24	21	33.3	42.9	54.6	58.1	0.0	0.0	8.538522	241	1344	1222-1222	1.000000	40.7
psbA_p3	psbA	3	TCAACGATTCATATACACTAAGACAA	TGAGATTGGTTGACATTGGCA	26	21	30.8	42.9	56.6	58.1	0.0	0.0	8.572492	241	1344	1223-1223	1.000000	40.7
psbA_p4	psbA	4	TTCAACGATTCATATACACTAAGACA	TGAGATTGGTTGACATTGGCA	26	21	30.8	42.9	56.6	58.1	0.0	0.0	8.572492	241	1344	1224-1224	1.000000	40.7
psbA_p5	psbA	5	TCAACGATTCATATACACTAAGACA	GAGATTGGTTGACATTGGCA	25	20	32.0	45.0	55.9	56.3	0.0	0.0	8.767473	241	1344	1222-1222	1.000000	40.7
rpoC1_p1	rpoC1	1	TGAAACTGGAGAGACCCGAA	TTGCTCGTTCGAGAACTACG	20	20	50.0	50.0	58.3	58.0	0.0	23.5	0.328197	21430	24248	2976-2977	1.000000	77.4
rpoC1_p2	rpoC1	2	TGAAACTGGAGAGACCCGAA	TTCGATTGCTCGTTCGAGAA	20	20	50.0	45.0	58.3	57.7	0.0	23.5	0.629262	21430	24248	2981-2982	1.000000	75.0
rpoC1_p3	rpoC1	3	TGAAACTGGAGAGACCCGAA	TTTCGATTGCTCGTTCGAGA	20	20	50.0	45.0	58.3	57.7	0.0	23.5	0.629262	21430	24248	2982-2983	1.000000	75.0
rpoC1_p4	rpoC1	4	TGAAACTGGAGAGACCCGAA	CGATTGCTCGTTCGAGAACT	20	20	50.0	50.0	58.3	58.4	0.0	23.5	0.669083	21430	24248	2979-2980	1.000000	74.7
rpoC1_p5	rpoC1	5	TGAAACTGGAGAGACCCGAA	TCGATTGCTCGTTCGAGAAC	20	20	50.0	50.0	58.3	58.4	0.0	23.5	0.673559	21430	24248	2980-2981	1.000000	74.6
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	48514	48861	438-438	1.000000	75.8
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGA	20	20	50.0	50.0	57.5	57.6	0.0	0.0	0.921818	48514	48861	299-400	1.000000	72.6
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	20	20	50.0	50.0	57.5	57.5	0.0	20.1	0.931776	48514	48861	300-435	1.000000	72.5
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.0	58.1	0.0	20.1	1.013432	48514	48861	427-427	1.000000	71.9
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	20	20	50.0	50.0	57.5	57.4	0.0	0.0	1.079322	48514	48861	410-410	1.000000	71.4
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	48935	49681	834-857	1.000000	80.7
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.254575	48935	49681	824-847	1.000000	80.2
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GGAAATGGTCGGGATAGCTC	TGAAAGTTGGATAGGCTGGC	20	20	55.0	50.0	57.8	57.9	0.0	0.0	0.300111	48935	49681	880-903	1.000000	79.7
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	48935	49681	877-900	1.000000	79.4
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	48935	49681	899-974	1.000000	79.3
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCCTTCACTAACCGGTGCTA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.1	57.8	8.5	0.0	0.269796	51373	53251	2017-2023	1.000000	78.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TAACCGGTGCTAAAACTCCG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	8.5	0.0	0.280450	51373	53251	2009-2015	1.000000	78.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCCTTCACTAACCGGTGCTA	AGCCCAATGCAACAAAATGC	20	20	50.0	45.0	58.1	58.5	8.5	0.0	0.546572	51373	53251	2099-2105	1.000000	75.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TAACCGGTGCTAAAACTCCG	AGCCCAATGCAACAAAATGC	20	20	50.0	45.0	57.9	58.5	8.5	0.0	0.557226	51373	53251	2091-2097	1.000000	75.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCCTTCACTAACCGGTGCTA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.1	57.5	8.5	0.0	0.613568	51373	53251	2016-2022	1.000000	75.3
accD_p1	accD	1	ATAGGGGGCAAGAAGACTCT	AGAAAACACCATCCTTTTTCGT	20	22	50.0	36.4	57.4	56.7	0.0	0.0	3.845011	59254	60768	1620-1620	1.000000	49.3
accD_p2	accD	2	ATAGGGGGCAAGAAGACTCT	TGTCATCAAAGAAAACACCATCC	20	23	50.0	39.1	57.4	57.5	0.0	0.0	4.029850	59254	60768	1629-1629	1.000000	47.8
accD_p3	accD	3	ATAGGGGGCAAGAAGACTCT	AAGAAAACACCATCCTTTTTCGT	20	23	50.0	34.8	57.4	57.4	0.0	0.0	4.169875	59254	60768	1621-1621	1.000000	46.7
accD_p4	accD	4	ATAGGGGGCAAGAAGACTCT	AGAAAACACCATCCTTTTTCGTT	20	23	50.0	34.8	57.4	57.4	0.0	0.0	4.169875	59254	60768	1620-1620	1.000000	46.7
accD_p5	accD	5	ATAGGGGGCAAGAAGACTCT	GTCATCAAAGAAAACACCATCCT	20	23	50.0	39.1	57.4	57.3	0.0	0.0	4.289923	59254	60768	1628-1628	1.000000	45.7
accD-psaI_p1	accD-psaI	1	CCACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	60769	61641	986-991	1.000000	79.6
accD-psaI_p2	accD-psaI	2	TCCACGGTTTCTTTCCCTTG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	60769	61641	987-992	1.000000	79.6
accD-psaI_p3	accD-psaI	3	CCACGGTTTCTTTCCCTTGA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	0.0	26.8	0.127877	60769	61641	992-997	1.000000	79.0
accD-psaI_p4	accD-psaI	4	TCCACGGTTTCTTTCCCTTG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	0.0	26.8	0.127877	60769	61641	993-998	1.000000	79.0
accD-psaI_p5	accD-psaI	5	TCAGCTCCACGGTTTCTTTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.1	58.0	0.0	26.9	0.148050	60769	61641	992-997	1.000000	78.8
rpl20-rps12_p1	rpl20-rps12	1	CGACGTTTCCGAGCTGTATA	TCTCCCGCTCTTAGAGGATG	20	20	50.0	55.0	57.5	58.0	0.0	11.5	0.475259	71125	71836	807-807	1.000000	76.2
rpl20-rps12_p2	rpl20-rps12	2	CGACGTTTCCGAGCTGTATA	GAGGAACATGCACTAGGGTG	20	20	50.0	55.0	57.5	58.0	0.0	0.0	0.493391	71125	71836	776-776	1.000000	76.1
rpl20-rps12_p3	rpl20-rps12	3	CGACGTTTCCGAGCTGTATA	GTCGAGGAACATGCACTAGG	20	20	50.0	55.0	57.5	58.1	0.0	0.0	0.535658	71125	71836	779-779	1.000000	75.7
rpl20-rps12_p4	rpl20-rps12	4	TCCGAGCTGTATATCCTCGT	TCTCCCGCTCTTAGAGGATG	20	20	50.0	55.0	57.4	58.0	14.4	11.5	0.574967	71125	71836	800-800	1.000000	75.4
rpl20-rps12_p5	rpl20-rps12	5	TCCGAGCTGTATATCCTCGT	GAGGAACATGCACTAGGGTG	20	20	50.0	55.0	57.4	58.0	14.4	0.0	0.593099	71125	71836	769-769	1.000000	75.3
clpP_p1	clpP	1	GGAATGGGTTGGTTTAGATCGA	AACCCATTGTTACGTTTCCA	22	20	45.5	40.0	58.1	55.5	0.0	0.0	4.648856	72087	74179	2263-2263	1.000000	42.8
clpP_p2	clpP	2	GGAATGGGTTGGTTTAGATCGA	AAACCCATTGTTACGTTTCCA	22	21	45.5	38.1	58.1	56.3	0.0	0.0	4.849867	72087	74179	2264-2264	1.000000	41.2
clpP_p3	clpP	3	GGAATGGGTTGGTTTAGATCG	AACCCATTGTTACGTTTCCA	21	20	47.6	40.0	56.7	55.5	0.0	0.0	4.860782	72087	74179	2263-2263	1.000000	41.1
clpP_p4	clpP	4	GGAATGGGTTGGTTTAGATCGA	ACCCATTGTTACGTTTCCAT	22	20	45.5	40.0	58.1	55.2	0.0	0.0	4.885563	72087	74179	2262-2262	1.000000	41.0
clpP_p5	clpP	5	TGGAATGGGTTGGTTTAGATCG	AACCCATTGTTACGTTTCCA	22	20	45.5	40.0	58.4	55.5	0.0	0.0	4.913858	72087	74179	2264-2264	1.000000	40.7
rpl16_p1	rpl16	1	TCAATGCAAAGGATGAAGGA	AGGTTGAATAAAAATTCGATTGACCA	20	26	40.0	30.8	54.9	58.1	0.0	0.0	9.192583	83489	85047	1706-1706	1.000000	40.0
rpl16_p2	rpl16	2	TCAATGCAAAGGATGAAGGA	GGTTGAATAAAAATTCGATTGACCA	20	25	40.0	32.0	54.9	56.9	0.0	0.0	9.198462	83489	85047	1705-1705	1.000000	40.0
rpl16_p3	rpl16	3	TCAATGCAAAGGATGAAGGAA	AGGTTGAATAAAAATTCGATTGACCA	21	26	38.1	30.8	55.7	58.1	0.0	0.0	9.360297	83489	85047	1706-1706	1.000000	40.0
rpl16_p4	rpl16	4	TTCAATGCAAAGGATGAAGGA	AGGTTGAATAAAAATTCGATTGACCA	21	26	38.1	30.8	55.7	58.1	0.0	0.0	9.360297	83489	85047	1707-1707	1.000000	40.0
rpl16_p5	rpl16	5	TCAATGCAAAGGATGAAGGAA	GGTTGAATAAAAATTCGATTGACCA	21	25	38.1	32.0	55.7	56.9	0.0	0.0	9.366175	83489	85047	1705-1705	1.000000	40.0
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	AGTCAGTTAACAGCCAACCG	GGGAGGGCGAATCTCATCTT	20	20	50.0	55.0	58.1	59.2	8.6	0.0	1.359245	111911	113311	1559-1641	1.000000	69.2
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	TCTACCACTGAGCTACTGGG	GGGAGGGCGAATCTCATCTT	20	20	55.0	55.0	57.9	59.2	8.3	0.0	1.383595	111911	113311	1538-1620	1.000000	69.0
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	ACGACCAGTCAGTTAACAGC	GGGAGGGCGAATCTCATCTT	20	20	50.0	55.0	57.8	59.2	8.6	0.0	1.388595	111911	113311	1565-1647	1.000000	68.9
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	AGGATTCGAACCTACGACCA	GGGAGGGCGAATCTCATCTT	20	20	50.0	55.0	58.2	59.2	15.0	0.0	1.394830	111911	113311	1578-1660	1.000000	68.9
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	GGATTCGAACCTACGACCAG	GGGAGGGCGAATCTCATCTT	20	20	55.0	55.0	57.8	59.2	0.0	0.0	1.443505	111911	113311	1577-1659	1.000000	68.5
ndhF-rpl32_p1	ndhF-rpl32	1	TTGTTGCTGTCGGAATAAGT	GGTGGAAACCGATTTCGCTA	20	20	40.0	50.0	55.3	58.3	0.0	0.0	2.973532	115550	115827	497-498	1.000000	56.4
ndhF-rpl32_p2	ndhF-rpl32	2	TTTGTTGCTGTCGGAATAAGT	GGTGGAAACCGATTTCGCTA	21	20	38.1	50.0	56.1	58.3	0.0	0.0	3.173323	115550	115827	498-499	1.000000	54.8
ndhF-rpl32_p3	ndhF-rpl32	3	TTTTGTTGCTGTCGGAATAAGT	GGTGGAAACCGATTTCGCTA	22	20	36.4	50.0	56.8	58.3	0.0	0.0	3.444863	115550	115827	499-500	1.000000	52.6
ndhF-rpl32_p4	ndhF-rpl32	4	TTTTTGTTGCTGTCGGAATAAGT	GGTGGAAACCGATTTCGCTA	23	20	34.8	50.0	57.5	58.3	0.0	0.0	3.778916	115550	115827	500-501	1.000000	49.9
ndhF-rpl32_p5	ndhF-rpl32	5	TGTTGCTGTCGGAATAAGTA	GGTGGAAACCGATTTCGCTA	20	20	40.0	50.0	54.4	58.3	0.0	0.0	3.846196	115550	115827	496-497	1.000000	49.4
psaC-ndhE_p1	psaC-ndhE	1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	120005	120469	659-663	1.000000	74.9
psaC-ndhE_p2	psaC-ndhE	2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	120005	120469	658-662	1.000000	74.9
psaC-ndhE_p3	psaC-ndhE	3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	20	20	50.0	50.0	58.1	58.6	0.0	12.2	0.757229	120005	120469	679-683	1.000000	74.9
psaC-ndhE_p4	psaC-ndhE	4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	20	20	50.0	55.0	58.1	58.8	0.0	6.0	0.891951	120005	120469	657-661	1.000000	73.8
psaC-ndhE_p5	psaC-ndhE	5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	20	20	55.0	50.0	58.3	57.4	0.0	0.0	0.943571	120005	120469	634-638	1.000000	73.5
ycf1_p1	ycf1	1	ACGCCATTAAATCCGTGTCA	TGTTCCCAGAATTTGTGCCA	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.181240	126547	127546	1159-1159	1.000000	78.6
ycf1_p2	ycf1	2	ACGCCATTAAATCCGTGTCA	CCCAGAATTTGTGCCACTCT	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.186929	126547	127546	1155-1155	1.000000	78.5
ycf1_p3	ycf1	3	ACGCCATTAAATCCGTGTCA	TGTGCCACTCTTTGATGCAT	20	20	45.0	45.0	57.9	58.1	0.0	0.0	0.188292	126547	127546	1146-1146	1.000000	78.5
ycf1_p4	ycf1	4	ACGCCATTAAATCCGTGTCA	TCCCAGAATTTGTGCCACTC	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.192370	126547	127546	1156-1156	1.000000	78.5
ycf1_p5	ycf1	5	ACGCCATTAAATCCGTGTCA	TAGAACCCCAGTTGTTCCCA	20	20	45.0	50.0	57.9	58.2	0.0	0.0	0.279645	126547	127546	1171-1171	1.000000	77.8