Hotspot

accD

LSC · 56934-58046 · 1113 bp

Back to Rhomboda

Pi 0.00719

MarkerSeek score 61.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1113
variable_sites: 8
pi: 0.00719
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TGAATAGTGGGTCTAATAGTAGT	TGATGTTCCCAAAGAAAACA	34.8	35.0	53.5	53.2	1265 bp	0.5	20.0
accD_p2	TGAATAGTGGGTCTAATAGTAGT	TGTTCCCAAAGAAAACATCA	34.8	35.0	53.5	53.2	1262 bp	0.5	20.0
accD_p3	TGAATAGTGGGTCTAATAGTAGT	TTGATGTTCCCAAAGAAAACA	34.8	33.3	53.5	54.1	1266 bp	0.5	20.0
accD_p4	TTGAATAGTGGGTCTAATAGTAGT	TGATGTTCCCAAAGAAAACA	33.3	35.0	54.3	53.2	1266 bp	0.5	20.0
accD_p5	TTGAATAGTGGGTCTAATAGTAGT	TGTTCCCAAAGAAAACATCA	33.3	35.0	54.3	53.2	1263 bp	0.5	20.0