Hotspot

rpl32-trnL-UAG

SSC · 111735-112546 · 812 bp

Back to Rhomboda

Pi 0.016

MarkerSeek score 59.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 812
variable_sites: 13
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTTTTCCAAAGATTTCTACGAA	TCCTAAGAGCAGCGTGTCTA	27.3	50.0	52.3	57.9	916-925 bp	1.0	41.2
rpl32-trnL-UAG_p2	TTTTTCCAAAGATTTCTACGAA	AGCAGCGTGTCTACCAATTT	27.3	45.0	52.3	57.8	909-918 bp	1.0	41.2
rpl32-trnL-UAG_p3	TTTTTCCAAAGATTTCTACGAA	CCTAAGAGCAGCGTGTCTAC	27.3	55.0	52.3	57.8	915-924 bp	1.0	41.2
rpl32-trnL-UAG_p4	TTTTTCCAAAGATTTCTACGAA	CTAAGAGCAGCGTGTCTACC	27.3	55.0	52.3	57.8	914-923 bp	1.0	41.2
rpl32-trnL-UAG_p5	TTTTTCCAAAGATTTCTACGAA	GAGCAGCGTGTCTACCAATT	27.3	50.0	52.3	58.3	910-919 bp	1.0	41.2