Hotspot

rpl16-rps3

LSC · 80335-81813 · 1479 bp

Back to Rhomboda

Pi 0.00884

MarkerSeek score 64.30

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1479
variable_sites: 13
pi: 0.00884
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CAAGTGCCTGAAGAGCGTAT	GCAGGGTTCCCTTACAAACA	50.0	50.0	58.0	58.0	1656-1660 bp	1.0	80.8
rpl16-rps3_p2	TCGCCCTGCTTCTATTTGTC	GCAGGGTTCCCTTACAAACA	50.0	50.0	58.0	58.0	1697-1701 bp	1.0	80.7
rpl16-rps3_p3	CGCCCTGCTTCTATTTGTCT	GCAGGGTTCCCTTACAAACA	50.0	50.0	58.0	58.0	1696-1700 bp	1.0	80.7
rpl16-rps3_p4	CTAGCTGTGATCCAAGCAGG	GCAGGGTTCCCTTACAAACA	55.0	50.0	58.0	58.0	1678-1682 bp	1.0	80.5
rpl16-rps3_p5	CTGTGATCCAAGCAGGTTCA	GCAGGGTTCCCTTACAAACA	50.0	50.0	57.8	58.0	1674-1678 bp	1.0	79.4