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psbN-psbH

LSC · 73022-73140 · 119 bp

Back to Rhomboda

Pi 0.0336

MarkerSeek score 71.80

Primer available yes

Label psbN-psbH

Feature Metadata

label_name: psbN-psbH
feature_type: igs
region: LSC
length_bp: 119
variable_sites: 4
pi: 0.0336
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbH_p1	GAAGATGGCGACTAGGGTTG	CCCTACGTCAGTTCGTCTTG	55.0	55.0	58.1	58.0	209 bp	1.0	80.9
psbN-psbH_p2	GAAGATGGCGACTAGGGTTG	CCTACGTCAGTTCGTCTTGG	55.0	55.0	58.1	58.0	208 bp	1.0	80.9
psbN-psbH_p3	GAAGATGGCGACTAGGGTTG	TCAGCGGTTTTTGTAGCCAT	55.0	45.0	58.1	58.1	169 bp	1.0	80.7
psbN-psbH_p4	GAAGATGGCGACTAGGGTTG	TCCCTACGTCAGTTCGTCTT	55.0	50.0	58.1	58.1	210 bp	1.0	80.3
psbN-psbH_p5	AACCGGATATGAAGATGGCG	CCTACGTCAGTTCGTCTTGG	50.0	55.0	57.8	58.0	218 bp	1.0	79.9