Hotspot

petA-psbJ

LSC · 62311-62760 · 450 bp

Back to Rhomboda

Pi 0.0111

MarkerSeek score 62.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 5
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	TATTCCGGGTTGGGTTCATC	50.0	50.0	58.0	57.3	562-566 bp	1.0	75.1
petA-psbJ_p2	GGCATCTGTGATTTTGGCAC	CTATTCCGGGTTGGGTTCAT	50.0	50.0	58.0	57.3	563-567 bp	1.0	75.0
petA-psbJ_p3	GATCCATTACGAGCCCAAGG	TATTCCGGGTTGGGTTCATC	55.0	50.0	58.1	57.3	597-601 bp	1.0	74.1
petA-psbJ_p4	GATCCATTACGAGCCCAAGG	CTATTCCGGGTTGGGTTCAT	55.0	50.0	58.1	57.3	598-602 bp	1.0	74.0
petA-psbJ_p5	GGCATCTGTGATTTTGGCAC	ATTCCGGGTTGGGTTCATCT	50.0	50.0	58.0	59.0	561-565 bp	1.0	72.8