Pi
0.00784
MarkerSeek score
61.60
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | TCAATCATGGAAAGAGCCGG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.0 | 58.0 | 1029-1085 bp | 1.0 | 84.2 |
| trnT-UGU-trnL-UAA_p2 | TCAATCATGGAAAGAGCCGG | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.0 | 58.1 | 1037-1093 bp | 1.0 | 83.6 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 972-1028 bp | 1.0 | 83.5 |
| trnT-UGU-trnL-UAA_p4 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 980-1036 bp | 1.0 | 82.9 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1004-1060 bp | 1.0 | 82.6 |