Hotspot

rps14-psaB

LSC · 36336-36471 · 136 bp

Back to Rhomboda

Pi 0.0147

MarkerSeek score 61.00

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 136
variable_sites: 2
pi: 0.0147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	CTCAACGAGGGAACTTTGCT	CCAGTGGCTCTTTCCATTGT	50.0	50.0	58.1	58.1	366 bp	1.0	78.8
rps14-psaB_p2	CTCAACGAGGGAACTTTGCT	TGGATTAGCCCACTTTTCCG	50.0	50.0	58.1	57.9	331 bp	1.0	78.6
rps14-psaB_p3	CTCAACGAGGGAACTTTGCT	GGATTAGCCCACTTTTCCGT	50.0	50.0	58.1	57.9	330 bp	1.0	78.6
rps14-psaB_p4	CTCAACGAGGGAACTTTGCT	TTGATTGCCTCCACATCAGG	50.0	50.0	58.1	57.9	282 bp	1.0	78.6
rps14-psaB_p5	TGTCGCTTCTTCTCCCTTTG	CCAGTGGCTCTTTCCATTGT	50.0	50.0	57.8	58.1	294 bp	1.0	78.5