Hotspot

trnG-UCC-trnfM-CAU

LSC · 35553-35793 · 241 bp

Back to Rhomboda

Pi 0.0373

MarkerSeek score 74.00

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 241
variable_sites: 9
pi: 0.0373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	AAAACGCGGATATGGTCGAA	GTTGTTTAGCGCGGAGTAGA	45.0	50.0	58.0	58.0	400-404 bp	1.0	81.6
trnG-UCC-trnfM-CAU_p2	AAAACGCGGATATGGTCGAA	CTTGAGGTCACGGGTTCAAA	45.0	50.0	58.0	58.0	351-355 bp	1.0	81.6
trnG-UCC-trnfM-CAU_p3	AGAAGATGCGGGTTCGATTC	GTTGTTTAGCGCGGAGTAGA	50.0	50.0	58.1	58.0	356-360 bp	1.0	81.4
trnG-UCC-trnfM-CAU_p4	GAGAAGATGCGGGTTCGATT	GTTGTTTAGCGCGGAGTAGA	50.0	50.0	58.1	58.0	357-361 bp	1.0	81.4
trnG-UCC-trnfM-CAU_p5	GTGTGGACATATGCGTACGT	GTTGTTTAGCGCGGAGTAGA	50.0	50.0	58.1	58.0	433-437 bp	1.0	80.9