Hotspot

psbM-trnD-GUC

LSC · 29072-30147 · 1076 bp

Back to Rhomboda

Pi 0.00856

MarkerSeek score 57.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1076
variable_sites: 9
pi: 0.00856
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CAGCGCAGTAGCAATAAACG	AGTTCAATCGGTTAGAGCACC	50.0	47.6	57.9	58.0	1180-1181 bp	1.0	73.5
psbM-trnD-GUC_p2	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTTAGAGCACC	45.0	47.6	58.3	58.0	1185-1186 bp	1.0	72.0
psbM-trnD-GUC_p3	CAGCGCAGTAGCAATAAACG	TGGCTGGGATTGTAGTTCAA	50.0	45.0	57.9	56.7	1193-1194 bp	1.0	71.5
psbM-trnD-GUC_p4	TGAACAGCGCAGTAGCAATA	AGTTCAATCGGTTAGAGCACC	45.0	47.6	57.6	58.0	1184-1185 bp	1.0	71.1
psbM-trnD-GUC_p5	AATGAACAGCGCAGTAGCAA	AGTTCAATCGGTTAGAGCACC	45.0	47.6	58.5	58.0	1186-1187 bp	1.0	70.3