Hotspot

atpH-atpI

LSC · 13175-13744 · 570 bp

Back to Rhomboda

Pi 0.0105

MarkerSeek score 64.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 570
variable_sites: 6
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTGGCTGCTG	45.0	45.0	57.9	58.3	673 bp	1.0	76.9
atpH-atpI_p2	CAGCAGCAATAACAGAAGCG	TTTTGCAACTTTGGCTGCTG	50.0	45.0	57.8	58.3	665 bp	1.0	76.7
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTGGCTGCT	45.0	40.0	57.9	57.6	674 bp	1.0	76.4
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	TTTTGCAACTTTGGCTGCTG	50.0	45.0	57.8	58.3	672 bp	1.0	76.4
atpH-atpI_p5	CAGCAGCAATAACAGAAGCG	TTTTTGCAACTTTGGCTGCT	50.0	40.0	57.8	57.6	666 bp	1.0	76.3