Hotspot

rps15

SSC · 127463-127735 · 273 bp

Back to Rhodoleia

Pi 0.00244

MarkerSeek score 28.50

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 1
pi: 0.00244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	AACACCATGCATTGATGGGT	GCACACATGCGTTGTCTTAC	45.0	50.0	58.1	58.0	759-760 bp	1.0	80.4
rps15_p2	TGCATTGATGGGTGATGAGG	GCACACATGCGTTGTCTTAC	50.0	50.0	57.9	58.0	752-753 bp	1.0	80.4
rps15_p3	TCTGTGCACCAATATCTGCC	GCACACATGCGTTGTCTTAC	50.0	50.0	58.0	58.0	1069-1070 bp	1.0	80.2
rps15_p4	TCTGTGCACCAATATCTGCC	TGGCAGTCGTTTCAGTATGG	50.0	50.0	58.0	57.9	1185-1186 bp	1.0	79.8
rps15_p5	TCTGTGCACCAATATCTGCC	GGCAGTCGTTTCAGTATGGT	50.0	50.0	58.0	57.9	1184-1185 bp	1.0	79.8