Hotspot

rpl20

LSC · 72550-72903 · 354 bp

Back to Rhodoleia

Pi 0.00377

MarkerSeek score 30.90

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 354
variable_sites: 2
pi: 0.00377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	AGCAATCTTTTCGTAGGCGT	GGCTGGGACAAAAGAAAGGA	45.0	50.0	57.9	58.0	1635-1659 bp	1.0	79.6
rpl20_p2	AGCAATCTTTTCGTAGGCGT	GCCAAAGGGTGTGAACTGTA	45.0	50.0	57.9	58.0	1256-1276 bp	1.0	79.6
rpl20_p3	TTGAAAGAACCGAGTCGACC	GGCTGGGACAAAAGAAAGGA	50.0	50.0	57.9	58.0	1419-1443 bp	1.0	79.3
rpl20_p4	CATTTTCCGGGGAACTCCAT	GGCTGGGACAAAAGAAAGGA	50.0	50.0	57.9	58.0	1157-1180 bp	1.0	79.0
rpl20_p5	ACTCTACCTTCCCGGAGTTC	GGCTGGGACAAAAGAAAGGA	55.0	50.0	58.2	58.0	1176-1199 bp	1.0	79.0