Hotspot

ndhD

SSC · 119911-121413 · 1503 bp

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Pi 0.00266

MarkerSeek score 46.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 6
pi: 0.00266
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GGCCTGACGAATACACACAT	GATACCCTGGGACGGATGTA	50.0	55.0	58.0	58.0	2045-2100 bp	1.0	82.6
ndhD_p2	GGCCTGACGAATACACACAT	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.0	58.0	2008-2063 bp	1.0	82.6
ndhD_p3	GCATGGGGCTCATATTGGAA	GATACCCTGGGACGGATGTA	50.0	55.0	58.0	58.0	2284-2339 bp	1.0	82.5
ndhD_p4	GCATGGGGCTCATATTGGAA	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.0	58.0	2247-2302 bp	1.0	82.5
ndhD_p5	GCATGGGGCTCATATTGGAA	TCCACGCGAATCCATTTGAT	50.0	45.0	58.0	58.0	2106-2161 bp	1.0	82.5