Hotspot

matK

LSC · 2129-3640 · 1512 bp

Back to Rhodoleia

Pi 0.00309

MarkerSeek score 60.70

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1512
variable_sites: 7
pi: 0.00309
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TCAACCCTTACTGCATGAACA	ACCTGTCTCCGAGGTATCTA	42.9	50.0	57.5	56.3	1740 bp	1.0	54.6
matK_p2	TCAACCCTTACTGCATGAACA	TACCTGTCTCCGAGGTATCT	42.9	50.0	57.5	56.3	1741 bp	1.0	54.6
matK_p3	TCAACCCTTACTGCATGAACA	TTACCTGTCTCCGAGGTATCT	42.9	47.6	57.5	57.1	1742 bp	1.0	52.7
matK_p4	TCAACCCTTACTGCATGAACA	CCTTGTTTTGACTGTATCGCA	42.9	42.9	57.5	56.8	1701 bp	1.0	51.0
matK_p5	TCAACCCTTACTGCATGAACA	ACCTTGTTTTGACTGTATCGC	42.9	42.9	57.5	56.8	1702 bp	1.0	51.0