Hotspot

accD

LSC · 60839-62305 · 1467 bp

Back to Rhodoleia

Pi 0.00273

MarkerSeek score 45.70

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1467
variable_sites: 6
pi: 0.00273
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GCGAATTTGACACGACATGG	AAATGGGGATTCTCGGGAGA	50.0	50.0	58.1	58.1	2099-2107 bp	1.0	79.0
accD_p2	GCGAATTTGACACGACATGG	TTGCCTTGCTTAATTTCGCG	50.0	45.0	58.1	58.0	2003 bp	0.333333	52.2
accD_p3	ACTCCTTCTACCCCATCCTG	TTGCCTTGCTTAATTTCGCG	55.0	45.0	57.8	58.0	2126 bp	0.333333	51.7
accD_p4	CTCCTTCTACCCCATCCTGT	TTGCCTTGCTTAATTTCGCG	55.0	45.0	57.8	58.0	2125 bp	0.333333	51.7
accD_p5	CGATGCGAATTTGACACGAC	TTGCCTTGCTTAATTTCGCG	50.0	45.0	58.2	58.0	2007 bp	0.333333	51.4