Hotspot

ndhH-rps15

SSC · 127363-127462 · 100 bp

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Pi 0.0539

MarkerSeek score 73.40

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 100
variable_sites: 8
pi: 0.0539
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	TGCATTGATGGGTGATGAGG	GTCAACGGCTACTGGCTTAT	50.0	50.0	57.9	57.7	262-263 bp	1.0	79.8
ndhH-rps15_p2	AACACCATGCATTGATGGGT	GTCAACGGCTACTGGCTTAT	45.0	50.0	58.1	57.7	269-270 bp	1.0	79.7
ndhH-rps15_p3	GAGTCGAAGAACACCATGCA	GTCAACGGCTACTGGCTTAT	50.0	50.0	58.2	57.7	278-279 bp	1.0	78.5
ndhH-rps15_p4	TGAGTCGAAGAACACCATGC	GTCAACGGCTACTGGCTTAT	50.0	50.0	58.2	57.7	279-280 bp	1.0	78.5
ndhH-rps15_p5	AACACCATGCATTGATGGGT	GGAAAACGTCAACGGCTACT	45.0	50.0	58.1	58.5	276-277 bp	1.0	78.2