Hotspot

petA-psbJ

LSC · 66935-67742 · 808 bp

Back to Rhodoleia

Pi 0.00909

MarkerSeek score 62.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 11
pi: 0.00909
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ACTGGTATTCCTGTGATCGG	50.0	50.0	58.0	56.7	1002-1021 bp	1.0	70.4
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	CTGGTATTCCTGTGATCGGT	50.0	50.0	58.0	56.7	1001-1020 bp	1.0	70.4
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	ACTGGTATTCCTGTGATCGG	50.0	50.0	57.8	56.7	985-1004 bp	1.0	69.5
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	CTGGTATTCCTGTGATCGGT	50.0	50.0	57.8	56.7	984-1003 bp	1.0	69.5
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	CTGGTATTCCTGTGATCGGT	50.0	50.0	58.2	56.7	998-1017 bp	1.0	69.3