Hotspot

ycf4-cemA

LSC · 64166-65068 · 903 bp

Back to Rhodoleia

Pi 0.00372

MarkerSeek score 50.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 903
variable_sites: 5
pi: 0.00372
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	AGAAGTGGAGTGAATGCTTTCT	45.0	40.9	57.9	57.6	954-961 bp	1.0	61.4
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	AGAAGTGGAGTGAATGCTTTCT	50.0	40.9	58.1	57.6	956-963 bp	1.0	61.1
ycf4-cemA_p3	AAAAAGCTGCTGAATTGGCC	AGAAGTGGAGTGAATGCTTTCT	45.0	40.9	57.8	57.6	981-988 bp	1.0	60.7
ycf4-cemA_p4	AAAGCTGCTGAATTGGCCTA	AGAAGTGGAGTGAATGCTTTCT	45.0	40.9	57.8	57.6	979-986 bp	1.0	60.3
ycf4-cemA_p5	TTTCTTGCGCGTACCAATTG	AGAAGTGGAGTGAATGCTTTCT	45.0	40.9	57.7	57.6	959-966 bp	1.0	59.5