Hotspot

psaA-ycf3

LSC · 44362-45026 · 665 bp

Back to Rhodoleia

Pi 0.00401

MarkerSeek score 61.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 4
pi: 0.00401
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGTTCTTGAGAAATGGCCGG	GAAAATCACGAGGCGTTTCG	50.0	50.0	58.5	58.1	795-817 bp	1.0	75.7
psaA-ycf3_p2	TTTTACTTCTGGTTCCGGCG	GAAAATCACGAGGCGTTTCG	50.0	50.0	58.5	58.1	723-745 bp	1.0	75.6
psaA-ycf3_p3	TGTTCTTGAGAAATGGCCGG	AATCACGAGGCGTTTCGAAT	50.0	45.0	58.5	58.3	792-814 bp	1.0	74.5
psaA-ycf3_p4	GTGTTCTTGAGAAATGGCCG	GAAAATCACGAGGCGTTTCG	50.0	50.0	57.4	58.1	796-818 bp	1.0	74.5
psaA-ycf3_p5	TTTTACTTCTGGTTCCGGCG	AATCACGAGGCGTTTCGAAT	50.0	45.0	58.5	58.3	720-742 bp	1.0	74.4