Hotspot

petN-psbM

LSC · 30306-31169 · 864 bp

Back to Rhodoleia

Pi 0.00694

MarkerSeek score 49.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 864
variable_sites: 9
pi: 0.00694
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATAGACAATCGGGAGACGGT	AATGGAAGTGGAAAAGCGGT	50.0	45.0	57.9	58.0	1821-1825 bp	1.0	80.1
petN-psbM_p2	TGTAATTCTCCCGATCCCGA	AATGGAAGTGGAAAAGCGGT	50.0	45.0	57.9	58.0	1840-1844 bp	1.0	80.0
petN-psbM_p3	AATCGGGAGACGGTAAGGAT	AATGGAAGTGGAAAAGCGGT	50.0	45.0	57.9	58.0	1815-1819 bp	1.0	80.0
petN-psbM_p4	ATCGGGAGACGGTAAGGATT	AATGGAAGTGGAAAAGCGGT	50.0	45.0	57.9	58.0	1814-1818 bp	1.0	80.0
petN-psbM_p5	TTCTCCCGATCCCGATAGAC	AATGGAAGTGGAAAAGCGGT	55.0	45.0	58.1	58.0	1835-1839 bp	1.0	79.9