Hotspot

atpH-atpI

LSC · 14821-15949 · 1129 bp

Back to Rhodoleia

Pi 0.000594

MarkerSeek score 59.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1129
variable_sites: 1
pi: 0.000594
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1217-1246 bp	1.0	76.1
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	1273-1302 bp	1.0	75.5
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	AGGCGAATCTATGGAGGGTC	45.0	55.0	58.0	58.7	1189-1218 bp	1.0	75.0
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	1218-1247 bp	1.0	74.9
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.2	58.5	1277-1306 bp	1.0	74.8