Hotspot

rrn16S-trnI-CAU

IRa · 203506-205962 · 2457 bp

Back to Rhododendron

Pi 0.0797

MarkerSeek score 78.50

Primer available yes

Label rrn16S-trnI-CAU

Feature Metadata

label_name: rrn16S-trnI-CAU
feature_type: igs
region: IRa
length_bp: 2457
variable_sites: 698
pi: 0.0797
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16S-trnI-CAU_p1	CCTCAGCCTACGGGGTATTA	CCCATTAGCATGCATCCAGT	55.0	50.0	58.0	58.0	2462-2904 bp	0.590909	75.5
rrn16S-trnI-CAU_p2	CCTCAGCCTACGGGGTATTA	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.0	58.0	2410-2852 bp	0.590909	75.5
rrn16S-trnI-CAU_p3	CCTCAGCCTACGGGGTATTA	GTCCCATTAGCATGCATCCA	55.0	50.0	58.0	58.0	2464-2906 bp	0.590909	75.5
rrn16S-trnI-CAU_p4	GGTATTAGCAGCCGTTTCCA	CCCATTAGCATGCATCCAGT	50.0	50.0	58.0	58.0	2449-2891 bp	0.590909	75.3
rrn16S-trnI-CAU_p5	GGTATTAGCAGCCGTTTCCA	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	2397-2839 bp	0.590909	75.3