Hotspot

rps16-rps19

IRa · 175810-176788 · 979 bp

Back to Rhododendron

Pi 0.0633

MarkerSeek score 82.90

Primer available yes

Label rps16-rps19

Feature Metadata

label_name: rps16-rps19
feature_type: igs
region: IRa
length_bp: 979
variable_sites: 269
pi: 0.0633
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-rps19_p1	ACAGAACAACCGATGTCGAG	CGTTGTTTTTCGACCGGAAG	50.0	50.0	57.9	58.0	1198-1530 bp	0.863636	85.9
rps16-rps19_p2	CTAGATCCTTGCCCCTGAGA	CGTTGTTTTTCGACCGGAAG	55.0	50.0	57.9	58.0	1317-1644 bp	0.863636	85.7
rps16-rps19_p3	ACAGAACAACCGATGTCGAG	ATTTGCGAAGTCCAGCGTAT	50.0	45.0	57.9	58.0	1498-1954 bp	0.727273	80.4
rps16-rps19_p4	CTAGATCCTTGCCCCTGAGA	ATTTGCGAAGTCCAGCGTAT	55.0	45.0	57.9	58.0	1612-2068 bp	0.727273	80.1
rps16-rps19_p5	ACAGAACAACCGATGTCGAG	TTGGAGAGATTCGCAATCCG	50.0	50.0	57.9	58.1	1489-1785 bp	0.636364	74.3