Hotspot

rps19-rps16

IRa · 172990-174679 · 1690 bp

Back to Rhododendron

Pi 0.0244

MarkerSeek score 71.40

Primer available yes

Label rps19-rps16

Feature Metadata

label_name: rps19-rps16
feature_type: igs
region: IRa
length_bp: 1690
variable_sites: 191
pi: 0.0244
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rps16_p1	CGGAAATTCGAAGTAGGTGC	AAGGCGGAGATTTTAAAAGA	50.0	35.0	56.9	52.8	1685-1898 bp	0.818182	43.0
rps19-rps16_p2	CGGAAATTCGAAGTAGGTGC	AGGCGGAGATTTTAAAAGAA	50.0	35.0	56.9	52.8	1684-1897 bp	0.818182	43.0
rps19-rps16_p3	CGGAAATTCGAAGTAGGTGC	AAAGGCGGAGATTTTAAAAGA	50.0	33.3	56.9	53.7	1686-1899 bp	0.818182	43.0
rps19-rps16_p4	CGGAAATTCGAAGTAGGTGC	AAGGCGGAGATTTTAAAAGAA	50.0	33.3	56.9	53.7	1685-1898 bp	0.818182	43.0
rps19-rps16_p5	CGGAAATTCGAAGTAGGTGC	AAAGGCGGAGATTTTAAAAGAA	50.0	31.8	56.9	54.5	1686-1899 bp	0.818182	43.0