Hotspot

rpl32-ndhF

IRb · 157227-157935 · 709 bp

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Pi 0.0453
MarkerSeek score 82.70
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
IRb
length_bp
709
variable_sites
120
pi
0.0453
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 AGCTTTTAAGGCTGCCCAAT AGGTATGATCCACGCATATTGA 45.0 40.9 58.1 57.1 810-843 bp 0.863636 62.7
rpl32-ndhF_p2 AGCTTTTAAGGCTGCCCAAT AAAAGGTATGATCCACGCAT 45.0 40.0 58.1 55.1 813-846 bp 0.863636 62.6
rpl32-ndhF_p3 AAAGCTTTTAAGGCTGCCCA AGGTATGATCCACGCATATTGA 45.0 40.9 58.3 57.1 812-845 bp 0.863636 60.9
rpl32-ndhF_p4 AAGCTTTTAAGGCTGCCCAA AGGTATGATCCACGCATATTGA 45.0 40.9 58.3 57.1 811-844 bp 0.863636 60.9
rpl32-ndhF_p5 AAGCTTTTAAGGCTGCCCAA AAAAGGTATGATCCACGCAT 45.0 40.0 58.3 55.1 814-847 bp 0.863636 60.8