Hotspot

trnI-CAU-rps16

IRb · 137967-142035 · 4069 bp

Back to Rhododendron

Pi 0.078

MarkerSeek score 76.80

Primer available yes

Label trnI-CAU-rps16

Feature Metadata

label_name: trnI-CAU-rps16
feature_type: igs
region: IRb
length_bp: 4069
variable_sites: 1153
pi: 0.078
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-rps16_p1	GTTGGGCGCTTTAACCATTC	GGATCCTCTTTCCCGAACAC	50.0	55.0	58.0	58.0	497-681 bp	0.636364	74.7
trnI-CAU-rps16_p2	CCCATTAGCATGCATCCAGT	GGATCCTCTTTCCCGAACAC	50.0	55.0	58.0	58.0	549-737 bp	0.636364	74.3
trnI-CAU-rps16_p3	GTCCCATTAGCATGCATCCA	GGATCCTCTTTCCCGAACAC	50.0	55.0	58.0	58.0	551-739 bp	0.636364	74.2
trnI-CAU-rps16_p4	AGAGATCCTCGTACATGGTGA	GGATCCTCTTTCCCGAACAC	47.6	55.0	57.7	58.0	460-644 bp	0.636364	64.8
trnI-CAU-rps16_p5	GGGCGATTAGATTCGAATCCA	GCGATCGTAGATTACCACCG	47.6	55.0	58.0	58.3	682-686 bp	0.227273	40.3