Hotspot

trnI-CAU-rpoB

LSC · 20706-22107 · 1402 bp

Back to Rhododendron

Pi 0.0854

MarkerSeek score 84.50

Primer available yes

Label trnI-CAU-rpoB

Feature Metadata

label_name: trnI-CAU-rpoB
feature_type: igs
region: LSC
length_bp: 1402
variable_sites: 518
pi: 0.0854
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-rpoB_p1	GTTGGGCGCTTTAACCATTC	CTTCATTTCCATCCCAGAGCA	50.0	47.6	58.0	58.0	991-2902 bp	0.954545	82.0
trnI-CAU-rpoB_p2	GTTGGGCGCTTTAACCATTC	TTCATTTCCATCCCAGAGCA	50.0	45.0	58.0	56.8	990-2901 bp	0.954545	80.5
trnI-CAU-rpoB_p3	AGTTGGGCGCTTTAACCATT	CTTCATTTCCATCCCAGAGCA	45.0	47.6	58.4	58.0	992-2903 bp	0.954545	79.1
trnI-CAU-rpoB_p4	TTGGGCGCTTTAACCATTCA	CTTCATTTCCATCCCAGAGCA	45.0	47.6	58.4	58.0	990-2901 bp	0.954545	79.1
trnI-CAU-rpoB_p5	GTTGGGCGCTTTAACCATTC	TCATTTCCATCCCAGAGCAT	50.0	45.0	58.0	56.6	989-2900 bp	0.954545	78.6