Hotspot

trnK-UUU-ycf3

LSC · 4400-6897 · 2498 bp

Back to Rhododendron

Pi 0.0267

MarkerSeek score 73.40

Primer available yes

Label trnK-UUU-ycf3

Feature Metadata

label_name: trnK-UUU-ycf3
feature_type: igs
region: LSC
length_bp: 2498
variable_sites: 330
pi: 0.0267
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-ycf3_p1	CGAATCCCTTGCTTCATCCA	GAAGCCGCTCAGTATTGGAA	50.0	50.0	58.0	58.0	2140-2624 bp	0.909091	87.8
trnK-UUU-ycf3_p2	ACGAATCCCTTGCTTCATCC	GAAGCCGCTCAGTATTGGAA	50.0	50.0	58.0	58.0	2141-2625 bp	0.909091	87.8
trnK-UUU-ycf3_p3	ATGGACGAATCCCTTGCTTC	GAAGCCGCTCAGTATTGGAA	50.0	50.0	58.0	58.0	2145-2629 bp	0.909091	87.8
trnK-UUU-ycf3_p4	GGACGAATCCCTTGCTTCAT	GAAGCCGCTCAGTATTGGAA	50.0	50.0	58.0	58.0	2143-2627 bp	0.909091	87.8
trnK-UUU-ycf3_p5	CGATGGTATGGACGAATCCC	GAAGCCGCTCAGTATTGGAA	55.0	50.0	57.9	58.0	2152-2636 bp	0.909091	87.5