Hotspot

ycf2

Genome · 47538-54383 · 6846 bp

Back to Rhodobryum

Pi 0.0391

MarkerSeek score 61.20

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6846
variable_sites: 268
pi: 0.0391
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GGATCTGCTTCACCCCAAAA	GCGTCAGTCTCTCTCCAAAA	50.0	50.0	58.1	57.6	612 bp	1.0	80.4
ycf2_p2	AATTGGATCTGCTTCACCCC	GCGTCAGTCTCTCTCCAAAA	50.0	50.0	57.9	57.6	616 bp	1.0	79.8
ycf2_p3	GGATCTGCTTCACCCCAAAA	TTTACGCGTCAGTCTCTCTC	50.0	50.0	58.1	57.1	617 bp	1.0	76.8
ycf2_p4	GGATCTGCTTCACCCCAAAA	TTACGCGTCAGTCTCTCTCC	50.0	55.0	58.1	58.9	616 bp	1.0	76.6
ycf2_p5	AATTGGATCTGCTTCACCCC	TTTACGCGTCAGTCTCTCTC	50.0	50.0	57.9	57.1	621 bp	1.0	76.1