Hotspot

ndhB

Genome · 82384-84538 · 2155 bp

Back to Rhodobryum

Pi 0.0375

MarkerSeek score 69.10

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: Genome
length_bp: 2155
variable_sites: 80
pi: 0.0375
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	CTCGAACCTCCACGCTTTAA	GCACAAGGAAAAGCTCTTGC	50.0	50.0	57.9	58.2	2743-2759 bp	1.0	81.1
ndhB_p2	CCATTTCACCATCAAGGCCT	GCACAAGGAAAAGCTCTTGC	50.0	50.0	58.1	58.2	2694-2710 bp	1.0	80.6
ndhB_p3	CTCGAACCTCCACGCTTTAA	CAAGACGCTTAGGTGGATCC	50.0	55.0	57.9	58.1	2809 bp	0.5	58.9
ndhB_p4	CCATTTCACCATCAAGGCCT	CAAGACGCTTAGGTGGATCC	50.0	55.0	58.1	58.1	2760 bp	0.5	58.4
ndhB_p5	ACCATTTCACCATCAAGGCC	CAAGACGCTTAGGTGGATCC	50.0	55.0	58.4	58.1	2761 bp	0.5	56.0