Hotspot

trnK-UUU-chlB

Genome · 58617-59292 · 676 bp

Back to Rhodobryum

Pi 0.0643

MarkerSeek score 82.20

Primer available yes

Label trnK-UUU-chlB

Feature Metadata

label_name: trnK-UUU-chlB
feature_type: igs
region: Genome
length_bp: 676
variable_sites: 43
pi: 0.0643
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-chlB_p1	AGTCGCACTTAAAAGCCGAG	GCGGCCAAAGAAGCTTTAAG	50.0	50.0	58.6	58.0	748-761 bp	1.0	80.6
trnK-UUU-chlB_p2	TAGTCGCACTTAAAAGCCGA	GCGGCCAAAGAAGCTTTAAG	45.0	50.0	57.3	58.0	749-762 bp	1.0	79.3
trnK-UUU-chlB_p3	TCGCACTTAAAAGCCGAGTA	GCGGCCAAAGAAGCTTTAAG	45.0	50.0	57.3	58.0	746-759 bp	1.0	79.3
trnK-UUU-chlB_p4	CGCACTTAAAAGCCGAGTAC	GCGGCCAAAGAAGCTTTAAG	50.0	50.0	57.2	58.0	745-758 bp	1.0	78.9
trnK-UUU-chlB_p5	ACCCTTGTCAATGGTTTGGA	GCGGCCAAAGAAGCTTTAAG	45.0	50.0	57.2	58.0	805-818 bp	1.0	78.7