Hotspot

trnE-UUC-trnY-GUA

Genome · 46939-47054 · 116 bp

Back to Rhodobryum

Pi 0.069

MarkerSeek score 75.20

Primer available yes

Label trnE-UUC-trnY-GUA

Feature Metadata

label_name: trnE-UUC-trnY-GUA
feature_type: igs
region: Genome
length_bp: 116
variable_sites: 8
pi: 0.069
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnY-GUA_p1	CTCTCTTTCAAGGAGGCGAC	TTTGAACCAGCGTAGGCATT	55.0	45.0	58.0	58.1	229 bp	1.0	82.0
trnE-UUC-trnY-GUA_p2	CTCTCTTTCAAGGAGGCGAC	TGGATTTGAACCAGCGTAGG	55.0	50.0	58.0	57.9	233 bp	1.0	81.9
trnE-UUC-trnY-GUA_p3	ATCGTCTAGTGGCCTAGGAC	TTTGAACCAGCGTAGGCATT	55.0	45.0	58.0	58.1	251 bp	1.0	81.7
trnE-UUC-trnY-GUA_p4	CATCGTCTAGTGGCCTAGGA	TTTGAACCAGCGTAGGCATT	55.0	45.0	58.0	58.1	252 bp	1.0	81.6
trnE-UUC-trnY-GUA_p5	ATCGTCTAGTGGCCTAGGAC	TGGATTTGAACCAGCGTAGG	55.0	50.0	58.0	57.9	255 bp	1.0	81.5