Hotspot

psbZ-trnS-UGA

Genome · 43057-43269 · 213 bp

Back to Rhodobryum

Pi 0.1

MarkerSeek score 75.90

Primer available yes

Label psbZ-trnS-UGA

Feature Metadata

label_name: psbZ-trnS-UGA
feature_type: igs
region: Genome
length_bp: 213
variable_sites: 21
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnS-UGA_p1	CCAACTGAAAGGCAATAGTCA	CATAAACCACTCGGCCATCT	42.9	50.0	56.2	57.7	255-259 bp	1.0	62.4
psbZ-trnS-UGA_p2	CCAACTGAAAGGCAATAGTCA	TAAACCACTCGGCCATCTCT	42.9	50.0	56.2	58.4	253-257 bp	1.0	61.6
psbZ-trnS-UGA_p3	CCAACTGAAAGGCAATAGTCA	TCAAGACCGGAGCCATAAAC	42.9	50.0	56.2	57.6	268-272 bp	1.0	61.5
psbZ-trnS-UGA_p4	CCAACTGAAAGGCAATAGTCA	GTTTTCAAGACCGGAGCCAT	42.9	50.0	56.2	58.5	272-276 bp	1.0	60.8
psbZ-trnS-UGA_p5	CCAACTGAAAGGCAATAGTCA	CAAGACCGGAGCCATAAACC	42.9	55.0	56.2	58.6	267-271 bp	1.0	59.7